EPS-G7 The Core Substrate for α-Amylase Assay Reagents

2026-08-27

The detection result of α-amylase is of great reference value for the diagnosis of various diseases, suchas acute pancreatitis, acute abdomen, chronic pancreatitis, etc. EPS-G7 is precisely the key substrate for this detection. According to the recommendation of the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC), ethylidene-4-NP-G7 should be used in diagnostic tests for the determination of α-amylase and pancreatic α-amylase.

EPS-G7 is 4,6-Ethylidene-4-nitrophenyl-1,4-α-D-maltoheptaoside, CAS 96597-16-9, molecular formula is C₅₀H₇₇NO₃₈, and molecular weight is 1300.1. This substrate is specifically designed for α-amylase activity measurement. The introduction of an ethylidene protecting group significantly enhances its specificity for α-amylase.

EPS-G7 is primarily used in α-amylase assay kits for clinical biochemistry. The core reaction principle is as follows: α-amylase in the sample hydrolyzes the α-1,4 glycosidic bonds of the substrate. Through a series of coupled enzymatic reactions (typically involving α-glucosidase), 4-nitrophenol (PNP) is ultimately released. By monitoring the rate of PNP production at 405 nm (kinetic method), the α-amylase activity in the sample can be accurately calculated.

By choosing our EPS-G7 substrate, you gain the following core advantages:

Extremely Low Blank Absorbance & Stable Blank Rate
Excellent Linear Range
Superior Stability
Strong Interference Resistance
Strict Batch-to-Batch Consistency

Choosing a high-purity, low-blank, kinetically superior EPS-G7 substrate is the foundation for developing high-quality α-amylase detection reagents. As a professional IVD raw material supplier, we offer batch-stable EPS-G7 products with complete Certificates of Analysis (COA) and support flexible, customized packaging specifications to help reagent manufacturers shorten development cycles and enhance product competitiveness.

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